Thymoquinone-releasing collagen/chitosan/nano-hydroxyapatite composite scaffold for enhanced bone regeneration.
Kazemi S., Jirofti N., Arabzadeh S., Mosaffa F., Hashemi M., Kalalinia F.
Laboratory Study, published in Int J Biol Macromol (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int J Biol Macromol (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40812656
- DOI
- 10.1016/j.ijbiomac.2025.146848
- Citations
- 2
Abstract (original English)
Objective Thymoquinone (TQ) has osteogenic properties. Loading TQ into scaffolds improves release, increases local bioavailability, and reduces toxicity. This study developed a TQ-loaded collagen-chitosan-nano hydroxyapatite (Col-Chi-nHAp) scaffold for bone regeneration. Methods Collagen, chitosan, and nano-hydroxyapatite were blended in acetic acid, followed by TQ (10, 25, and 50 μM) loading and cross-linked using glutaraldehyde. Scaffolds were fabricated via freeze-drying. Physicochemical properties were assessed through scanning electron microscopy (SEM), shrinkage, degradation rate, porosity, water absorption, mechanical testing, Fourier-transform infrared spectroscopy (FTIR), and TQ release rate. Biocompatibility was evaluated via Human adipose-derived mesenchymal stem cells (ASC) proliferation, while osteoconductivity was determined by alkaline phosphatase (ALP) activity. Results The scaffold exhibited a porous microstructure with an average pore size of 85 ± 3.23 μm, porosity of 96 ± 1.93 %, and water absorption capacity of 5368 ± 181.46 %, with minimal shrinkage or degradation. TQ (25 μM)-loaded scaffold revealed a significantly higher Young's modulus than other groups in Mechanical testing. Sustained TQ release was observed over a 21 days. The 3D scaffold environment enhanced ASC proliferation 4.2-fold over monolayers, underscoring its biomimetic potential, and improve
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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