Level D· Preclinical Evidence

Nanofat grafting: basic research and clinical applications

Tonnard P., Verpaele A., Peeters G., Declercq H.

Reports 67 clinical cases of nanofat for superficial rhytides, scars and dark lower eyelids, plus analysis of the cellular content of processed fat.

Study type
Case Series
Journal
Plastic and Reconstructive Surgery (2013)
Country
Belgium
Sample size
67
Follow-up
Not systematically reported
PMID
23783059
DOI
10.1097/PRS.0b013e31829fe1b0

What was studied

Injection of emulsified and filtered fat (nanofat) through needles as fine as 27 gauge.

Methods

  • Compared three fat samples: macrofat, microfat and nanofat.
  • Analysed adipocyte viability and cells from the stromal vascular fraction.

Key findings

  • Nanofat contained almost no viable adipocytes but retained SVF cells.
  • Established the concept that effects come from SVF cells rather than volume augmentation.

Limitations

  • Uncontrolled case series without standardised outcome measures.
  • Published in 2013; techniques have evolved considerably.

What this study does not prove

  • Does not prove comparative efficacy against other methods.
  • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • Limited follow-up cannot indicate long-term outcomes.

Clinical interpretation

A landmark paper underpinning nanofat/SVF facial use, but low-level evidence.

Evidence level: Preclinical Evidence

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