Tracking in vitro biodegradation dynamics in cartilage tissue engineering using dual-labeled hydrogel/scaffold composites.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

This study addresses the challenges of tracking cell-mediated biodegradation in cartilage tissue engineering, where hydrogels and scaffolds play a crucial role in providing structural support and promoting tissue regeneration. This research area has been rarely studied, offering potential insights into bridging the gap between in vitro and in vivo conditions for real-time monitoring of tissue regeneration alongside biodegradation. We developed dual-labeled hydrogel/scaffold composites for real-time monitoring of scaffold degradation in response to cell activity. Gelatin methacryloyl (GelMA) hydrogels are extensively explored for cartilage tissue engineering, albeit concerns remain regarding their mechanical properties under load-bearing conditions. To address this, a hydrogel/scaffold composite system was employed in this study, where a poly ( ϵ -caprolactone) (PCL) hex prism edge structure acts as a scaffold to support the cell-laden GelMA hydrogel. Fluorophore labeling of GelMA and PCL facilitated non-invasive monitoring of the hydrogel/scaffold composite biodegradation under cell proliferation conditions. Initially, the behavior of fluorescent-tagged Hydrogel/Scaffold was examined under accelerated degradation conditions. Subsequently, human adipose-derived mesenchymal stem cells loaded into fluorescent-labeled hydrogel/scaffolds were evaluated for their biocompatibility pot

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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