Transcriptional plasticity of stromal cells amplifies their differentiation efficiency in vitro.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Human bone marrow-derived stromal cells (also termed mesenchymal stem cell) are progenitors capable of differentiating into bone-forming osteoblasts and fat-storing adipocytes. Due to the loss of bone mass being associated with increased marrow fat, trans-differentiation of osteoblasts into adipocytes has been hypothesized as a contributor to osteoporotic bone loss and fragility. Reprogramming of transcriptional networks is a prerequisite for cellular differentiation; however, to which extent cell type-specific transcriptional networks modulate cellular plasticity within stromal cells remains unknown. In this study, we performed gene expression analysis at bulk and single cell level in stromal cells being repeatedly exposed to osteogenic and adipogenic inducers in vitro. Surprisingly, cell type-specific gene networks are not suppressive but instead promoting to obtain an opposing phenotype, for example, enhanced osteoblast differentiation of adipogenic prestimulated stromal cells compared to undifferentiated ones. Mechanistically, lineage-selective genes with enhanced response upon interconversion are primed in the stem cell state and obtain modest activity levels during exposure to opposing lineage conditions. Finally, the presence of cells simultaneously shows an osteogenic and adipogenic phenotype highlighting a strong molecular plasticity of transcriptional networks in stro

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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