Transcriptome analysis of mRNA after different induction time of induced Schwann-like cells from adipose-derived stem cells.
Lin Z., Zhang Z., Qiu Q., Lu J., Su S., Wong CW.
Animal Study, published in Int J Surg (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Int J Surg (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40505047
- PMCID
- PMC12430886
- DOI
- 10.1097/JS9.0000000000002716
Abstract (original English)
Objectives To compare the differences in protein-coding transcripts before and after different induction times of adipose-derived stem cells (ADSCs)-induced Schwann-like cells (iSCs). Methods ADSCs were isolated from healthy adult female rats. In addition, the iSCs of 7 and 19 days after induction were chosen for ribonucleic acid (RNA)-sequencing (RNA-seq). Bioinformatic analysis was applied to determine the differences among ADSCs (group 1, g1), iSCs-7d (group 2, g2), and iSCs-19d (group 3, g3). Eight differentially expressed messenger RNAs (DEmRNAs) were randomly selected for quantitative real-time polymerase chain reaction to verify the accuracy of sequencing data. Results Compared with g1, g2, and g3 had 83 and 189 DEmRNAs, respectively. DEmRNAs of g2 were located in the synapse area and enriched in the nucleotide-binding oligomerization domain-like signaling pathway through Kyoto Encyclopedia of Genes and Genomes analysis. Located in the neuromuscular junction and extracellular matrix (ECM), DEmRNAs of g3 were enriched in the cellular response to cyclic adenosine monophosphate and the development of the peripheral nervous system through Gene Ontology analysis. Venn analysis showed that 31 genes were up-regulated after induction, and their protein products were mainly located in the ECM and enriched in the Janus kinase-signal transducer and activator of transcription (JAK/S
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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