Transcriptomic plasticity of cholinergic adipose macrophages in the acute thermogenic response
Knights AJ., Kim EJ., Liu S., Wu J.
Animal Study, published in J Biol Chem (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Biol Chem (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41207622
- PMCID
- PMC12719665
- DOI
- 10.1016/j.jbc.2025.110925
- Citations
- 2
Abstract (original English)
Cholinergic adipose macrophages (ChAMs) have recently been shown to regulate the acute thermogenic response in subcutaneous white adipose tissue, yet their transcriptomic dynamics are poorly understood, and little is known about their origins or identity. Using single-cell RNA sequencing (scRNAseq), we profiled ChAT-eGFP+ cells (expressing choline acetyltransferase) from subcutaneous white adipose tissue of mice housed at thermoneutrality or after acute cold exposure. We identified twelve distinct clusters of ChAT-expressing cells, predominated by hematopoietic cell types. Specifically, ChAMs exhibited increased proportions and Chat expression after acute cold. Widespread differential gene expression was induced in ChAMs after cold compared to thermoneutrality, with cold-enriched pathways in immune signaling, chemotaxis, and metabolism. Several ChAM subsets were uncovered that resembled previously reported adipose macrophage subpopulations. ChAMs were predicted to have mixed origins, derived from adult bone marrow and embryonically. These findings provide a high granularity assessment of cholinergic immune cells in fat, and we highlight the transcriptomic plasticity and mixed origins of ChAMs, suggesting their therapeutic potential for metabolic diseases.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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