Transcriptomics Analysis of the Adipogenic Differentiation Mechanism of Bovine Adipose-Derived Neural Crest Stem Cells.
Zhang K., Tang X., Zhao R., Yan Y., Song X.
Laboratory Study, published in Animals (Basel) (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Animals (Basel) (2025)
- Country
- Switzerland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40867681
- PMCID
- PMC12383184
- DOI
- 10.3390/ani15162353
- Citations
- 1
Abstract (original English)
The aim of the present study was to investigate the expression of related genes during the differentiation process of baNCSCs into adipocytes using transcriptomics technique, thereby clarifying the potential mechanism underlying baNCSCs differentiation into adipocytes and providing insights into lipid metabolism and regulation of lipid deposition in ruminants. Transcriptomic analysis was conducted on the adipocytes of baNCSCs on days 0 (CON0), 3 (DIF3), and 9 (DIF9) of differentiation. The results showed that in the early stage of adipocyte differentiation of baNCSCs, differentially expressed genes (DEGs) are mainly involved in metabolic pathways such as chromosome modification, cell cycle progression, and regulation of stem cell pluripotency. In the middle and late stages of differentiation, DEGs are mainly involved in metabolic pathways such as changes in cell morphology and synthesis of fatty acids and triglycerides. Predicting the top 10 core hub genes (CHGs) in the protein-protein interaction (PPI) network that regulate various differentiation stages of adipocytes reveals that ERBB2 , EGFR , and MYC are upregulated during the early differentiation stage. In contrast, ITGB1 , KRAS , CCND1 , ACTB , VEGFA , MET , and HRAS are downregulated. During the middle and late stages of differentiation, the expressions of TP53 , CASP3 , STAT3 , CTNNB1 , JUN , EGFR , and MYC are upregul
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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