Transplantation of adipose-derived stem cell aggregates via hydrogel microspheres that incorporate growth factors increases muscle strength.
Abe T., Tanaka Isomura E., Kuwahara T., Mitsui R., Matsukawa M., Nakagawa K.
Laboratory Study, published in Regen Ther (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Regen Ther (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40678343
- PMCID
- PMC12268345
- DOI
- 10.1016/j.reth.2025.05.016
Abstract (original English)
Introduction Stem cell transplantation is widely employed to treat various diseases, and adipose-derived mesenchymal stem cells (ASCs) are used for allogeneic xenotransplantation. However, muscle function post stem cell transplantation remains largely understudied. Therefore, we aimed to investigate the optimal conditions for the transplantation of ASC aggregates of gelatin hydrogel microparticles incorporating growth factors. We further aimed to establish a method for improving muscle function via ASC implantation combined with muscle loading through treadmill running. Methods Mouse ASCs suspended in various solutions were transplanted into the soleus muscles. The strength of each mouse was measured using a digital force gauge after a muscle load was applied using a treadmill. Results Platelet-rich growth factor-BB (PDGF-BB) effectively facilitated the expression of MYO-D in the ASCs. Moreover, the injection of cell aggregates rather than suspensions enhanced cell retention. Transplantation of ASC-aggregate gelatin hydrogel microparticles incorporating PDGF-BB in combination with muscle load using a treadmill enhanced mouse muscle function. Conclusions ASC aggregates with growth factor transplantation likely enhance cell retention. Moreover, they likely improve muscle function and load. Thus, our findings provide new avenues for cell regeneration therapy in muscle rehabilitati
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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