[Transplanted adipose-derived stem cells promote the expression of deleted in colorectal cancer (DCC) and neurofilament-200 (NF-200) in peri-infarct cortex of rats].
Huang H., Jiang J., Zeng C., Chen Y., Mei A.
Animal Study, published in Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2019)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31223108
Abstract (original English)
Objective To investigate the effects of adipose-derived stem cells (ADSCs) transplantation on the expression of deleted in colorectal cancer (DCC) and neurofilament-200 (NF-200) in peri-infarct cortex of rats. Methods SD rats were randomly divided into sham group, model group and ADSCs group. The rat model of middle cerebral artery occlusion (MCAO) was established with the modified Longa's method. Twenty-four hours after MCAO, 50 μL of cell suspension containing about 2×10 6 PKH-26-labeled ADSCs was injected into the carotid artery of rats in the ADSCs group, and the same dose of saline was given to the rats in the model group. Day 7 and 14 after MCAO, the migration of ADSCs in rat brains was observed under a fluorescence microscope, and the protein levels of DCC and NF-200 in the peri-infarct cortex were measured by Western blot analysis and the expression and distribution of DCC and NF-200 were detected by immunofluorescent histochemistry. Results PKH-26-labeled ADSCs were observed in the rat brains of ADSC group, and most of them were located in the peri-infarct cortex. At 7 and 14 days after MCAO, the expression of DCC in the model group was higher than that in the sham group, while the expression level of NF-200 was lower. Besides, the expression of DCC and NF-200 in the ADSC group was both higher than that in the model group. In the peri-infract cortex, DCC was mainly exp
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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