Transplants of fresh and cryopreserved autologous adipose tissue improve reintegrative phenomena in newly formed muscle tissue in the reconstruction of muscle volume loss: A histological evaluation.
Cantarero I., Peña-Toledo MA., Lopez-Espejo ME., Gil-Belmonte MJ., Villalba R., Jimena I.
Animal Study, published in Anat Rec (Hoboken) (2026) — summary generated from the PubMed abstract.
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- Study type
- Animal Study
- Journal
- Anat Rec (Hoboken) (2026)
- Country
- United States
- Reported sample size
- —
- PMID
- 40557468
- DOI
- 10.1002/ar.70010
Abstract (original English)
Repair of volumetric muscle loss (VML) lesions tends to be poor, leading to fibrosis and functional loss. Here we investigated the effectiveness of fresh versus cryopreserved autologous adipose tissue transplantation to facilitate repair. Wistar rats were distributed into four experimental groups: normal control, VML lesions, VML lesions treated with a fresh autologous adipose tissue graft, and VML treated with a cryopreserved autologous adipose tissue graft. At 60 days post-intervention, a histological, histochemical, and immunohistochemical analysis was performed to assess changes related to the degree of fibrosis, vascularization, and innervation. These changes were quantified and statistically analyzed. The changes generated by the two types of adipose tissue implanted in the VML lesion were highly similar, with some significant differences favoring the use of cryopreserved adipose tissue. Compared with the VML group, the following outcomes were observed: (1) significant presence of regenerated muscle fibers; (2) significant reduction of fibrosis, albeit with a higher proportion of type III collagen; (3) significant increase in the microvascular pattern; and (4) significant reduction in the number of angulated atrophic muscle fibers and increase in cytoarchitectural changes indicative of reinnervation. This study suggests that autologous adipose tissue transplantation in VM
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence comes from animal or laboratory studies and has not been confirmed in humans.
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