Tunable, Photoreactive Hydrogel System To Probe Synergies between Mechanical and Biomolecular Cues on Adipose-Derived Mesenchymal Stem Cell Differentiation.
Banks JM., Harley BAC., Bailey RC.
Laboratory Study, published in ACS Biomater Sci Eng (2015) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- ACS Biomater Sci Eng (2015)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33435093
- DOI
- 10.1021/acsbiomaterials.5b00196
- Citations
- 13
Abstract (original English)
The incorporation of the photoreactive molecule benzophenone into polyacrylamide hydrogels, allowing for orthogonal control over spatial incorporation of biomolecules and selective modulation of matrix stiffness, is described. Adipose-derived mesenchymal stem cells were cultured on matrices whose elastic moduli are tuned to ∼5, ∼14, and ∼37 kPa combined with an immobilized growth factor, bone morphogenic protein 2 (BMP-2), and their lineage differentiation was determined. BMP-2 was observed to have the most pronounced effect at the intermediate stiffness, while the lowest and highest stiffness hydrogels are directed by elasticity alone. Together, this approach describes a facile platform for fundamental studies of cell fate decisions in the context of both mechanical and biochemical cues and may lead to improved insight and effectiveness of stem cell therapies.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.