Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Nanovesicles Alleviated Colitis via Modulating Th17/Treg Balance Through Hsa-miR-27b-3p-Mediated Suppression of PI3K/AKT/STAT3 Signaling Pathway

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract, characterized by persistent immune dysregulation. Umbilical cord mesenchymal stem cell-derived extracellular nanovesicles (MSC NVs) exhibit immunomodulatory properties, demonstrating significant therapeutic potential for clinical applications. This study sought to investigate the therapeutic effects of MSC NVs against colitis and elucidate the underlying mechanisms. Methods MSC NVs were prepared from umbilical cord MSCs using a continuous filtration-extrusion method. The therapeutic effects of MSC NVs were assessed by tail vein injection in a murine model of DSS-induced colitis. Results MSC NVs significantly markedly ameliorated colitis-associated symptoms, including body weight loss, colon length reduction, and elevated disease activity index scores. MSC NVs not only mitigated colitis-induced intestinal barrier impairment and inflammatory responses, but also exhibited targeted biodistribution to inflamed colonic lesions. Unexpectedly, administration of MSC-NVs via the tail vein significantly altered the gut microbial composition in colitic mice, particularly enhancing the relative abundances of beneficial commensal genera Lachnoclostridium and Dubosiella , consequently reestablishing microbial homeostasis. Moreover, MSC NVs modulated the T help (Th) 17/ regulatory T (T

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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