Unveiling the dental cellular landscape: A comprehensive review of single-cell RNA sequencing in human dental tissues
Shah MS., Kondo T., Sundarabupathi M., Samaranayake LP., Jamal M., Egusa H.
Systematic Review, published in Jpn Dent Sci Rev (2025) — summary generated from the PubMed abstract.
Relatively higher-quality human studies compared with other topics in this database, e.g. multiple RCTs or systematic reviews. This does not mean it is standard or approved care.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Systematic Review
- Journal
- Jpn Dent Sci Rev (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41377691
- PMCID
- PMC12686620
- DOI
- 10.1016/j.jdsr.2025.11.004
- Citations
- 2
Abstract (original English)
Single-cell RNA sequencing (scRNA-seq) has advanced the understanding of cellular heterogeneity and molecular dynamics in human dental tissues. This systematic review critically evaluated scRNA-seq studies exploring the cellular composition of human dental tissues, comparing in situ and cultured environments under both healthy and diseased conditions. Original research articles meeting the inclusion criteria were assessed for cell type identification, transcriptional profiles, analytical platforms, and other key biological insights by two independent authors. A total of fifteen studies that met the inclusion criteria revealed diverse cell populations, including mesenchymal stem cells (MSCs), immune cells, endothelial cells, and odontoblast-like cells, each exhibiting distinct transcriptional signatures in homeostasis, regeneration, and disease. Key findings included distinct MSC subpopulations, including THY1 + , CD24 + , FRZB + , and NOTCH3 + / PDGFRB + , exhibiting unique differentiation potential, along with significant heterogeneity in odontoblast lineage. Moreover, carious dental pulp undergoes a fibrotic transcriptional shift (e.g., COL1A1 , FN1 , and TNC ), whereas periodontitis exhibits increased expression of pro-inflammatory cytokines ( IL-1β and TNFα) and diminished periodontal MSC regenerative capacity. This review underscores the contributions of scRNA-seq in provi
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Relatively higher-quality human studies compared with other topics in this database, e.g. multiple RCTs or systematic reviews. This does not mean it is standard or approved care.
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