VEGF-neutralized platelet-rich plasma with adipose-derived stromal vascular fraction enhanced osteochondral repair in a point-of-care goat model.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background/objective Focal articular cartilage defects do not heal spontaneously. Platelet-rich plasma (PRP) is as an autologous source of growth factors that has been reported to promote tissue healing. However, PRP contains angiogenic factors such as vascular endothelial growth factor (VEGF) that are known to inhibit chondrogenesis and promote osteoarthritis progression. Mesenchymal stem cells (MSCs) have also shown promise in promoting focal cartilage repair, but MSC therapies typically require cell isolation and expansion before implantation in a second surgery. Adipose-derived stromal vascular fraction (SVF) is rich in MSCs and can be harvested and delivered in a single procedure. The purpose of this study was to determine the effect of VEGF-neutralized PRP on in vitro chondrogenesis and in vivo osteochondral repair when combined with an MSC-rich SVF in a single-stage point-of-care goat model. It was hypothesized that VEGF-neutralized PRP would enhance in vitro chondrogenesis and improve in vivo osteochondral repair when combined with SVF. Methods Adipose-derived MSCs (ASCs) were encapsulated in photocrosslinkable hydrogels supplemented with (1) Hanks' Balanced Salt Solution (HBSS), (2) PRP, (3) VEGF-neutralized PRP, or (4) transforming growth factor- β3 (TGF-β3) for in vitro experiments. Cell viability and gene expression were determined on days 3, 7, and 21. For in vivo

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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