Level D· Scientific groundwork from lab and animal studiesLaboratory StudyPubMed

In Vitro Expansion of Human Adipose-Derived Stem Cells (ASCs).

Adam LT., Ambele MA., Pepper MS.

Laboratory Study, published in Methods Mol Biol (2025) — summary generated from the PubMed abstract.

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Level D· Scientific groundwork from lab and animal studiesEvidence level of this study

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
Read the A–D evidence level guide

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Laboratory Study
Journal
Methods Mol Biol (2025)
Country
United States
Reported sample size
—
Source database
PubMed
PMID
40445284
DOI
10.1007/978-1-0716-4607-6_11

Abstract (original English)

Adipose-derived stromal/stem cells (ASCs) have raised a great deal of interest in research and regenerative medicine with potential applications in the treatment of several diseases. ASCs are often required in large numbers for use in research and clinical applications. Standardization of isolation methods, expansion, and characterization will ensure quality of the cells as well as safety in their use. This protocol describes the expansion and characterization of ASCs and highlights ASC viability assessment following expansion.

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

How we grade evidence
HumansAdipose TissueCell Culture TechniquesStem CellsCell DifferentiationCell ProliferationCell SurvivalCells, CulturedMesenchymal Stem CellsCell Separation

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