In vitro exploration of the therapeutic potential of mesenchymal stem cell conditioned medium in endometriosis treatment by targeting apoptosis and cellular migration.
Hedayatnia H., Kalhor N.
Laboratory Study, published in Int J Reprod Biomed (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int J Reprod Biomed (2026)
- Country
- Iran
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42051963
- DOI
- 10.18502/ijrm.v24i2.21052
Abstract (original English)
Endometriosis, a common gynecological disorder involving ectopic endometrial tissue, leads to infertility and chronic pain. Dysregulated apoptosis and abnormal cell migration are key pathological features. Given current treatment limitations, novel strategies like mesenchymal stem cells (MSC) derived conditioned media (CM) are explored due to their rich secretome. To investigate the effects of CM from healthy menstrual blood-derived MSCs (MenSCs-CM) and human adipose tissue-derived MSCs (ADSCs-CM) on apoptosis and migration in endometriotic MenSCs. This in vitro study involved the isolation of endometriotic MenSCs from infertile women (25-35 yr) via menstrual blood. Mononuclear cells were cultured to passage 3, and characterized using flow cytometry (CD29, CD44, CD73, CD105 positive; CD34, CD38, CD45 negative). CM was prepared separately from healthy donor MenSCs and ADSCs. Endometriotic MenSCs were treated with healthy MenSCs-CM and ADSCs-CM independently. Cell migration (scratch assay), apoptosis (Annexin V), and Bax mRNA levels and Bax/Bcl-2 ratio (real-time polymerase chain reaction) were evaluated. Both ADSCs-CM and MenSCs-CM significantly increased during early and late apoptosis in endometriotic MenSCs (p < 0.001). Scratch assays showed significantly decreased MenSCs migration at 24, 48, and 72 hr (p < 0.001, p = 0.003, p < 0.001, respectively). Gene expression revealed
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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