In Vitro Tool: 3D Cell Culture of Human Adipose Tissue-Derived Mesenchymal Stromal Cells on Low Stiffness Silicone Scaffolds.
Stock P.
Laboratory Study, published in Methods Mol Biol (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Methods Mol Biol (2021)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33687686
- DOI
- 10.1007/978-1-0716-1225-5_19
Abstract (original English)
Conventional two-dimensional as well as established three-dimensional cell culture systems exhibit a high modulus of elasticity, which largely do not represent the stiffness of human organs. This chapter describes a protocol to establish 3D cell cultures of human adipose tissue-derived mesenchymal stromal cells (hAT-MSC) on low stiffness silicon scaffolds. These feature a defined growing environment with a stiffness close to physiology, thus being useful for in vitro investigations for multiple applications.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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