In vivo imaging of adipose-derived stem cell sheets on biodegradable nonwoven fabric using X-ray CT.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background A biodegradable nonwoven fabric that can be used to extract adipose-derived stem cells (ADSCs) from adipose tissue slices was developed, which were cultured rapidly without enzymatic treatment. The extracted and cultured ADSCs remain on the nonwoven fabric and form a thick cell sheet. The aim was to use the thick cell sheet as a treatment by transplanting it into the living body. In addition, the expectation was that it will be possible to observe the cell sheet in the living body using X-ray computed tomography (CT) because the nonwoven fabric used to produce the cell sheet contains 50% (by weight) hydroxyapatite. Results Thick cell sheets of ADSCs supported by two layers of nonwoven fabric were cut to size and transplanted into the cheeks of rats. No health damage was observed in the rats in which the cell sheets were implanted, except for one in which the surgery appeared to have failed. X-ray CT imaging showed that the fabric of the implanted cell sheet biodegraded over 12 weeks. Changes in the position, shape, and size of the cell sheet within the rat's body were tracked by X-ray CT. The thick cell sheets, which can be easily produced by simply seeding tissue slices, can be cut into appropriate shapes and transplanted safely, and it was confirmed that they slowly biodegraded when transplanted into the rats' bodies. Conclusions We demonstrated not only that the t

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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