WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice
Lin B., Wang C., Yang Y., Liu Z., Mu P., Xu W.
Animal Study, published in Adipocyte (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Adipocyte (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41292047
- PMCID
- PMC12667666
- DOI
- 10.1080/21623945.2025.2588888
Abstract (original English)
Objective Brown adipose tissue activation is a potential anti-obesity strategy. N 7 -methylguanosine (m 7 G) modification is a novel RNA epigenetic modification, but its role in adipose metabolism remains unexplored. Methods Male mice were fed a high-fat diet (HFD), followed by PCR array screening. Gain-of-function experiments and TRAC-seq were employed to explore WDR4 function. Result A Mouse Epigenetic Modification Enzymes PCR Array revealed that WDR4 expression showed the most pronounced downregulation in HFD mice. Overexpression of WDR4 in 3T3-L1 cells and primary adipocytes significantly increased UCP1 expression and suppressed lipid droplet formation, and enhanced mitophagy as evidenced by mitochondrial ultrastructure, autophagic vesicles, and LC3 expression. Suppression of mitophagy using 3-MA and bafilomycin A1 attenuated WDR4-induced adipocyte browning. WDR4 overexpression enhanced translational activity and reshaped the tRNA m 7 G methylome in 3T3-L1 adipocytes, specifically induced 38 unique tRNA m 7 G modification sites, and increasing cleavage scores of multiple tRNAs. GSE229240 dataset revealed that WDR4 mutation significantly reduced translation efficiency of 195 genes enriched in the TGF-β signalling , including BMP8B. Knockdown of BMP8B partially counteracted WDR4-mediated mitophagy. Conclusion WDR4 promotes adipocyte browning by enhancing BMP8B translation thr
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.