WTAP/YTHDF1-mediated m<sup>6</sup>A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs
Chen Q., Ao L., Zhao Q., Tang L., Xiong Y., Yuan Y.
Animal Study, published in J Adv Res (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Adv Res (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 38944238
- PMCID
- PMC12126738
- DOI
- 10.1016/j.jare.2024.06.019
- Citations
- 16
Abstract (original English)
Introduction The immunosuppressive capacity of mesenchymal stem cells (MSCs) is dependent on the "license" of several pro-inflammatory factors to express immunosuppressive molecular profiles, which determines the therapeutic efficacy of MSCs in immune-mediated inflammatory diseases. Of those, interferon-γ (IFN-γ) is a key inducer for the expression of immunosuppressive molecular profiles; however, the mechanism underlying this effect is unknown. Objectives To elucidate the regulation mechanism and biological functions of N 6 -methyladenosine (m 6 A) modification in the immunosuppressive functions by the IFN-γ-licensing MSCs. Methods Epitranscriptomic microarray analysis and MeRIP-qPCR assay were performed to identify the regulatory effect of WTAP in the IFN-γ-licensing MSCs. RIP-qPCR, western blot, qRT-PCR and RNA stability assays were used to determine the regulation of WTAP/m 6 A/YTHDF1 signaling axis in the expression of immunosuppressive molecules. Further, functional capacity of T cells was tested using flow cytometry, and both DSS-induced colitis mice and CIA mice were constructed to clarify the effect of WTAP and YTHDF1 in MSC-mediated immunosuppression. Results We identified that IFN-γ increased the m 6 A methylation levels of immunosuppressive molecules, while WTAP deficiency abolished the IFN-γ-induced promotion of m 6 A modification. IFN-γ activated ERK signaling, wh
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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