Xenogenic induction of adipose tissue and maintenance through pre- and post-conditioning using external volume expansion.
Wu M., Matar DY., Yu Z., Karvar M., Chen Z., Ng B.
Animal Study with a reported sample of 17, published in Biomed Mater (2022) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Biomed Mater (2022)
- Country
- England
- Reported sample size
- 17
- Source database
- PubMed
- PMID
- 36126655
- DOI
- 10.1088/1748-605X/ac934b
- Citations
- 2
Abstract (original English)
External volume expansion (EVE) has been shown to improve fat graft survival. In this study, we investigated the xenogenic implantation of human allograft adipose matrix (AAM) in non-immunocompromised mice in combination with pre- and post-conditioning with EVE to assess long-term adipose tissue survival. Sixty-eight recipient sites in thirty-four eight-week-old wild type (C57BL/6J) mice were separated into four groups. Thirty-four sites received no conditioning and either a subcutaneous injection of 300 μl saline ( n = 17; PBS group) or AAM ( n = 17; AAM group). Thirty-four sites received pre-conditioning with EVE (Day -7-3 pre-grafting) and 300 μl of AAM. Seventeen of these sites received immediate post-conditioning (Day 1-5 post-grafting) and 17 delayed post-conditioning (Day 28-32 post-grafting). Tissue was harvested at week 12 for analysis. At 12 weeks, immediate and delayed post-conditioning enabled higher volume retention ( p = 0.02 and p < 0.0001, respectively). Adipose Stem Cells were greater in the AAM+Del-EVE group compared to the AAM ( p = 0.01). Microvessel density was lower in the AAM group compared to the AAM+Imm-EVE ( p = 0.04) and AAM+Del-EVE group ( p = 0.02). Macrophage infiltration was lower in the AAM+Imm-EVE ( p = 0.002) and AAM+Del-EVE ( p = 0.003) groups compared to the AAM group. PCR analysis and Western blotting identified a significantly higher expres
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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