Zinc Sulphate Mediates the Stimulation of Cell Proliferation of Rat Adipose Tissue-Derived Mesenchymal Stem Cells Under High Intensity of EMF Exposure.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Unlike the role of mesenchymal stem cells (MSCs) in regenerative medicine, their application in cell therapy can be complicated by factors such as a reduction in the proliferation potential, senescent tendency of MSCs to expand, and an age-dependent decline in their number and functions. It was shown that there is an association between exposure to electromagnetic fields (EMFs) and response to stress, cell proliferation, aging, and cell death. Furthermore, the zinc ion, as an essential trace element, was reported to be involved in the regulation of the growth and cell proliferation. In this report, ratadipose tissue-derived mesenchymal stem cells (rADSCs) were randomly divided into four groups-group I (control without any ZnSO 4 and EMF); group II (ZnSO 4 treatment without EMF exposed); group III (EMF exposed without ZnSO 4 ); and group ІV (EMF exposed with ZnSO 4 )-to evaluate whether 0.14 μg/ml Zinc sulfate (ZnSO 4 ) could affect cell proliferation of rADSCs under extremely low frequency-electromagnetic field (ELF-EMF). The methyl thiazoltetrazolium (MTT) method was used to determine the cell proliferation of rADSCs following exposure to ELF-EMF in the presence of ZnSO 4 . The immunocytochemistry method as well as flow-cytometry was used to identify the cell surface markers. Next, oil red O, alizarin red, toluidine blue, and cresyl violet staining was done to evaluate the adi

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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