106 Adipose Derived Stem Cell Paracrine Factor Release in Smokers vs Non Smokers with Burn Injuries

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Abstract Introduction Adipose-derived stem cells (ADSCs) have an important role in the modulation of burned tissue repair through the release of paracrine factors that stimulate the wound healing response. In this study, we tested the hypothesis that smoking status alters the profile of paracrine factors secreted from ADSCs isolated from damaged adipose tissue. Methods Adipose tissue was collected from adult patients (N=8) with severe burn injuries ( >20% total body surface area) at the index operation. ADSCs were extracted and cultured in vitro. Supernatants were harvested 30 hours after plating and used for cytokine determinations by Multiplex assay. Fluorescence activated single cell sorting (FACS) confirmed their phenotype with markers CD 90, CD 166, and CD 73. Univariate analyses were performed to compare the two cohorts (Smokers vs non smokers). All outliers were removed. Results Higher amounts of anti-inflammatory cytokines IL-4(p=0.03) and IL-10(p=0.04) and pro-inflammatory cytokines TNF-alpha (0.03), IL-8, and IFN-gamma (p=0.03) were detected in burn patients who were current everyday smokers when compared to nonsmokers, or former smokers. No significant differences in supernatant concentrations of IL-17, IL-1 beta, TGF-alpha, IL-6, and IL-13 were observed (p >0.05). Mortality was higher in the smoker group when compared to non-smokers (p=0.02). Conclusions The results

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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