AB237. Human adipose derived stem cells induced cell apoptosis and S phase arrest in bladder tumor

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Mesenchymal stem cells (MSCs) had received much attention in recent years owing to their capacity to differentiate intaao many other cell types. Currently a lot of studies found that MSCs could secrete cytokines and chemokines which affect the growth of tumor cells. Adipose derived mesenchymal stem cells (ADSCs) could differentiate into a variety of different cells as well which were available easier. They could affect the growth of tumor cells, but the mechanism was not clear. We were trying to investigate their effect on bladder cancer cells growth to illustrate the possible mechanisms. Methods Transwell chamber co-culture was used to observe the effect of ADSCs on bladder cancer cell line T24 and EJ growth. T24 and EJ cells were cultured with condition medium of ADSCs and then recorded tumor cell activity by MTS assay. We used AnnexinV-PI double staining for tumor cell apoptosis, colony formation assay for tumor cell proliferation, wound healing for tumor cell migration, western blot for 5 detecting tumor cell associated protein expressing and molecular signaling pathways. Results The cell counting and colony formation assay showed ADSCs inhibited the proliferation of EJ and T24 cells. Cell viability assessment revealed that the secretions, in the form of conditioned medium, were able to decrease cancer cell viability. Wound-healing assay suggested ADSCs suppresse

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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