Adipose-derived mesenchymal stem cells from solid tissue and lipoaspirates: A comparative study of phenotype, growth, and secretome.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Mesenchymal stem cells (MSCs) are considered a promising therapy for various diseases due to their strong potential in regenerative medicine and immunomodulation. The tissue source of MSCs has gained attention for its role in influencing their function, accessibility, and readiness for clinical use. To identify the most suitable adipose source for MSC isolation and expansion for further applications. We isolated MSCs from solid adipose tissue and liposuction aspirates using the enzyme method. The MSCs were examined for their expansion using population doubling time, differentiation capacity using multilineage differentiation induction, surface markers using flow cytometry, and stability of chromosomes using the karyotyping method. Growth factors and cytokines in MSC-conditioned media were analyzed using the Luminex assay. MSCs were isolated from solid adipose tissue and lipoaspirates and expanded from passage 0 to passage 2. All adipose-derived MSCs (AD-MSCs) exhibited the typical elongated, spindle-shaped morphology and comparable proliferation rate. They expressed positive surface markers [cluster of differentiation 73 (CD73): > 97%, CD90: > 98%, and CD105: > 95%], and negative markers (< 1%). All MSCs expressed similar levels of stemness genes (octamer-binding transcription factor 4, SRY-box 2, Krüppel-like factor, and MYC), colony-forming, and trilineage differentiation pot

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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