Analysis of the (Lymph-)Angiogenic Potential of Regenerative Scaffolds and the Impact of Adipose-Derived Mesenchymal Stem Cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Regenerative matrices are essential in the therapy of complex wounds, where lymphangiogenesis and angiogenesis play crucial roles. This work investigates the lymph and angiogenic potential of regenerative scaffolds and the influence of adipose-derived mesenchymal stem cells (ADSC) in a rodent wound model. Methods In a wound model, the authors administered different acellular dermal matrices (ADMs) to 2 wounds on each rat-enriching 1 with ADSCs and leaving the other without. ADSCs were isolated from rodent inguinal fat tissue, characterized using fluorescence-activated cell sorting and their viability confirmed in vitro on different matrices. Tissue samples were collected on days 7, 14, 21, and 28 postoperatively. Six different ADMs, including 2 synthetic and 4 biological variants, were compared using histologic, immunofluorescence staining, and molecular biological analyses. Results ADM integration varied, with collagen (COL)-elastin-ADM showing the highest integration (95%) and polyurethane (PU)-ADM, the lowest (23%) after 7 days. Synthetic ADMs, particularly PU-ADM, exhibited the highest mRNA expression of angiogenesis and lymphangiogenesis markers at 7 and 28 days. Protein analysis showed that synthetic ADMs had the highest CD31 levels from 14 to 28 days, whereas COL-ADM had the highest LYVE1 and PROX1 levels at 21 and 28 days. Immunofluorescence revealed polylact

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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