SVF
Level C· Early Human ResearchProspective Study

Anti-Inflammatory and Angiogenic Effects of Stem Cell Secretome.

Grogan SP., Stinebaugh G., D'Lima DD.

Prospective Study on Osteoarthritis, Chronic Inflammation, published in Int J Mol Sci (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Prospective Study
Journal
Int J Mol Sci (2026)
Country
Switzerland
Reported sample size
—
PMID
41828549
DOI
10.3390/ijms27052325

Abstract (original English)

Mesenchymal stem cells (MSCs) exert biological effects in part through their secretome which includes extracellular vesicles. In this study, we isolated and characterized the secretome from clinically relevant stem cell lines: human embryonic stem cell-derived mesenchymal stem cell line (ES-MSCs) and Infrapatellar fat pad derived MSC (IPFP-MSC) cultured in xeno-free medium. We assessed the biological activity of concentrated cell secretome or isolated fractions of extracellular vesicles (EVs) on cell proliferation, microvascular formation, and cartilage degradation in a human osteoarthritic (OA) ex vivo model. Serum-free conditioned medium from ES-MSC ( N = 1) or IPFP-MSC ( N = 2) monolayer cultures were concentrated by ultrafiltration to generate concentrated conditioned medium (CCM). Size exclusion chromatography was used to fractionate extracellular vesicles (EVs). Vesicle size, concentration, morphology, and surface markers were characterized by nanoparticle tracking analysis, transmission electron microscopy, and flow cytometry. Biological activity was evaluated by treating human umbilical vein endothelial cells (HUVECs), IPFP-MSCs, and ES-MSCs with CCM and EVs at defined particle concentrations. Endothelial network formation was tested in fibrin gels with different cell and secretome combinations. For analysis of cartilage degradation, human cartilage explants ( N = 4; 3.

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.

Evidence level

Early human evidence such as case series or small samples, often without a control group.

How we grade evidence
HumansSecretomeMesenchymal Stem CellsExtracellular VesiclesCulture Media, ConditionedHuman Umbilical Vein Endothelial CellsCell ProliferationOsteoarthritisAngiogenesisNeovascularization, Physiologic
Face & SkinKneeOsteoarthritisChronic Inflammation
View on PubMedDOI

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