Asymmetry in traction forces produced by migrating preadipocytes is bounded to 33.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Wound healing by gap closure is accomplished by the migratory cells within tissues. In fat tissue, the preadipocytes, stem cells committed to the adipose (fat) lineage, typically migrate into the wound area, and then differentiate into mature adipocytes to facilitate tissue repair and regeneration. While cell migration has previously been characterized, typically for fibroblasts, little is known about the dynamic, mechanical interactions of migrating cells with their microenvironment; cells crawl on a two-dimensional (2D) substrate by attaching and applying forces that allow them to extend leading edges and retract their rear. Moreover, preadipocytes, the highly migratory precursors of fat cells, have not been studied from this aspect. Here, we have evaluated the migration of preadipocytes, through their speed and directionality as well as the magnitude of the lateral forces applied during their migration on a 2D gel with Young's modulus of 2.44 kPa. We have found that the preadipocytes migrate non-directionally in the absence of chemoattractant, at an average rate of 0.27 µm/min, similar to fibroblasts. The preadipocytes exhibited a wide range of total traction forces (100-800 nN), and migrated along the long axis of their elongated morphology. Interestingly, we have observed an asymmetry in the location of force application between the lead and rear of the cells that was boun

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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