SVF
Level D· Scientific groundwork from lab and animal studiesAnimal StudyPubMed

Biofabricated Adipose-Derived Mesenchymal Cell Sheets Recover Cryo-Injured Kidneys in Rats.

Kitahara R., Imamura T., Domen T., Matsumoto Y., Inoue Y., Ogawa N.

Animal Study with a reported sample of 14 on Chronic Kidney Disease, published in Tissue Eng Part A (2024) — summary generated from the PubMed abstract.

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Level D· Scientific groundwork from lab and animal studiesEvidence level of this study

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
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This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
Tissue Eng Part A (2024)
Country
United States
Reported sample size
14
Source database
PubMed
PMID
39276109
DOI
10.1089/ten.TEA.2024.0164

Abstract (original English)

This study aimed to develop a treatment for chronic kidney disease (CKD) by investigating whether transplantation of biofabricated adipose-derived mesenchymal cell (AMC) sheets could improve renal tissue and function. Thirty-nine ten-week-old male Sprague-Dawley rats underwent the harvesting of adipose tissues and right nephrectomy. AMCs that were collected from adipose tissues were labeled and cultured on temperature-responsive dishes and applied to a gelatin hydrogel sheet. Subsequently, two identical AMC-gelatin sheets were attached together to biofabricate a bilayered AMC-gelatin sheet. Furthermore, 3 weeks after nephrectomy, the renal artery and vein of the left kidney were clamped, and the kidney was sprayed with liquid nitrogen for 60 s. The biofabricated AMC sheet was autologously transplanted into the renal capsule of the cryo-injured region ( n = 14). Control rats were given acellular sheet ( n = 25). One day before and 4 weeks after transplantation, blood and 24-h urinary specimens were collected. Histological analysis of the experimental kidneys was performed 4 weeks after transplantation. Four weeks after transplantation, in the acellular control-transplanted rats, creatinine clearance levels tended to increase, and serum creatinine levels significantly increased. However, in the biofabricated AMC sheet-transplanted rats, creatinine clearance levels significantly i

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

How we grade evidence
AnimalsMaleRats, Sprague-DawleyKidneyMesenchymal Stem CellsAdipose TissueRatsMesenchymal Stem Cell Transplantation
Systemic / IVChronic Kidney Disease
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