SVF
Level D· Preclinical EvidenceLaboratory Study

A biomimetic folding paper co-culture system for analyzing cellular crosstalk in bone healing: Interactions of bone morphogenetic protein-2, Wnt family member 1, periostin, and platelet-derived growth factor subunit A be

Prawira F., Alexandra C., Tong YW., Hartrianti P., Rindiarti A., Chen AC.

Laboratory Study on Hip, published in Talanta (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Laboratory Study
Journal
Talanta (2026)
Country
Netherlands
Reported sample size
—
PMID
41045659
DOI
10.1016/j.talanta.2025.128943

Abstract (original English)

Bone defects, commonly associated with osteoporosis, result in fragile bones prone to fractures in both men and women. Osteoporotic fractures often lead to prolonged healing due to impaired cell differentiation. Understanding cellular crosstalk during bone regeneration is crucial for developing effective treatments. Periosteum-derived progenitor cells (PDPCs) and adipose-derived stem cells (ADSCs) play essential roles in bone formation because ADSCs secret key growth factors, such as bone morphogenetic protein-2, Wnt family member 1, Periostin, and platelet-derived growth factor subunit A, that promote osteogenesis. To investigate cellular interactions in bone healing process in the natural bone microenvironment, we developed a biomimetic folding paper co-culture system that mimics the inflamed three-dimensional bone structure. This system enables co-culture of PDPCs and ADSCs, allowing the study of their protein crosstalk and osteogenic potential. Moreover, neutralizing assays were conducted to inhibit specific cytokines and evaluate their influence on osteogenesis. Our findings confirm that ADSCs promote osteogenesis through their secreted growth factors that enhance the differentiation and activity of PDPCs. Disrupting these cellular interactions through cytokine inhibition led to a significant reduction in osteogenic potential. It was evidenced by decreased protein expressi

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
Coculture TechniquesHumansBone Morphogenetic Protein 2PeriosteumAdipose TissueStem CellsCell Adhesion MoleculesOsteogenesisPlatelet-Derived Growth FactorCell Differentiation
Hip
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