Debrided skin as a source of autologous stem cells for wound repair.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Major traumatic injuries to the body, such as large surface area burns, limit the availability of autologous stem cell populations for wound repair. This report demonstrates that even after severe burn trauma to the body, resident stem cells present within the subcutaneous adipose tissue survive and are available for therapeutic uses. Debrided skin from wounded areas contains subcutaneous adipose tissue and can yield approximately 1.5 × 10(5) to 2.5 × 10(5) cells per milliliter of tissue. This observation indicates that tissue, which is normally discarded, could be a valuable source of stem cells. Initial immunohistochemistry of the debrided tissue localized platelet-derived growth factor receptor beta(+) (PDGFR-β(+) ) cells to perivascular niches of vascular beds. It was immunophenotypically confirmed that the cell isolates are stem cells and designated as debrided skin adipose-derived stem cells (dsASCs). Gene expression analysis of stem cell specific transcripts showed that the dsASCs maintained their stemness over serial passages. Furthermore, dsASCs were able to differentiate into adipogenic, osteogenic, and vascular cell lineages. Finally, an in vivo excision wound model in athymic rats demonstrated that the dsASCs are engrafted within a wound bed after 12 days. These data provide the first evidence that subcutaneous adipose tissue from discarded burned skin contains a vi

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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