Discrete adipose-derived stem cell subpopulations may display differential functionality after in vitro expansion despite convergence to a common phenotype distribution.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Complex immunophenotypic repertoires defining discrete adipose-derived stem cell (ASC) subpopulations may hold a key toward identifying predictors of clinical utility. To this end, we sorted out of the freshly established ASCs four subpopulations (SPs) according to a specific pattern of co-expression of six surface markers, the CD34, CD73, CD90, CD105, CD146, and CD271, using polychromatic flow cytometry. Method Using flow cytometry-associated cell sorting and analysis, gating parameters were set to select for a CD73 + CD90 + CD105 + phenotype plus one of the four following combinations, CD34 - CD146 - CD271 - (SP1), CD34 - CD146 + CD271 - (SP2), CD34 + CD146 + CD271 - (SP3), and CD34 - CD146 + CD271 + (SP4). The SPs were expanded 700- to 1000-fold, and their surface repertoire, trilineage differentiation, and clonogenic potential, and the capacity to support wound healing were assayed. Results Upon culturing, the co-expression of major epitopes, the CD73, CD90, and CD105 was maintained, while regarding the minor markers, all SPs reverted to resemble the pre-sorted population with CD34 - CD146 - CD271 - and CD34 - CD146 + CD271 - representing the most prevalent combinations, followed by less frequent CD34 + CD146 - CD271 - and CD34 + CD146 + CD271 - variants. There was no difference in the efficiency of adipo-, osteo-, or chondrogenesis by cytochemistry and real-time

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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