Effect of Eucomis autumnalis on the Osteogenic Differentiation of Adipose-Derived Stem Cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Eucomis autumnalis subsp. autumnalis (Mill.) Chitt. ( EASA ) is a commonly used medicinal plant for the treatment of fractures, osteoarthritis, back pain, and wound healing in Southern Africa. In this study, the effects of water and acetone extracts of EASA on the viability, osteogenic differentiation, and mineralization of human adipose-derived stem cells (hADSCs) were investigated in vitro . The results showed that both water and acetone extracts of EASA increased cell viability at concentrations between 10 and 50 μg/mL on day 7 and 14 of treatment. Osteogenic differentiation and mineralization of hADSCs were optimal at 5 μg/mL for the water extract and at 5-10 μg/mL for the acetone extract. A 5 μg/mL acetone extract upregulated the expression of the ALP, Runx2, Col1a1 , and osteopontin genes. In addition, EASA upregulated β-catenin, cyclin D1 , and osteoprotegerin ( OPG ). The results suggest that EASA may likely upregulate the expression of β-catenin , which subsequently upregulates the osteogenic marker genes through Runx2 . However, EASA also upregulates cyclin D1 supporting the growth of precursor cells. Additionally, EASA upregulated the expression of OPG suggesting that it may inhibit bone resorption. The results of this study support the traditional use of the plant in bone healing. Impact statement Herbal remedies are used to treat various ailments by almost 80% of t

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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