The effect of neuropeptides kyotorphin and neokyotorphin on proliferation of cultured brown preadipocytes.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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Abstract (original English)

Stimulation of DNA and protein synthesis in brown preadipocytes by 1 microM neokyotorphin in serum-containing media was comparable with the effect of 1 microM norepinephrine. In serum-free medium a decrease and a shift of the maximal effect to lower concentration of neokyotorphin were observed. Kyotorphin had no effect on cell proliferation in either medium; however, 0.01-1 microM kyotorphin inhibited the cell proliferation stimulated by 1 microM norepinephrine. Norepinephrine and both peptides stimulated comparable Ca2+ rise in freshly isolated brown preadipocytes. The effects of neokyotorphin and norepinephrine were additive, whereas 0.03-0.3 microM kyotorphin blocked the action of 3 microM norepinephrine. The peptides did not affect the cAMP level in non-stimulated or norepinephrine-stimulated cultured cells. The effects of the peptides on the brown fat cell cultures indicate that peripheral tissue cells contain receptors for these neuropeptides.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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