[Effects of adipose-derived stem cells-hyaluronic acid composite on healing of wound combined with radiation injury].

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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• Emerging · Emerging topic under active research

Abstract (original English)

To observe and explore the effects of adipose-derived stem cells (ADSCs)-hyaluronic acid (HA) composite on healing of wound combined with radiation injury. The ADSCs were harvested from the fat tissue in groin of 10 inbred Sprague Dawley (SD) rats and were isolated and cultured by enzyme digestion. The ADSCs-HA composite was prepared with ADSCs (5 x 10(6) cells/mL) at passage 6 and HA (10 mg/mL). Thirty inbred SD rats, 15 males and 15 females, were randomly divided into groups A (n = 10), B (n = 10), and C (n = 10). A 2 cm x 2 cm full-thickness skin defect was made on the rat back before 20 Gy 60Co radiation exposure. One week after debridement, wounds were treated by petrolatum gauze in group A as the control group, by HA (0.4 mL) and petrolatum gauze in group B, and by ADSCs-HA composite (0.4 mL) and petrolatum gauze in group C. The microvessel density (MVD) and the distribution of CD90 positive cells were observed at 1st, 2nd, 3rd, and 4th weeks. The wound healing was slower, and wound did not heal at 4th week and still filled with granulation tissue in group A; the wound healing of group B was faster than that of group A, and the wound did not heal completely with depression in the center at 4th week; the wound healed completely with epidermilizated surface and no obvious depression at 4th week in group C. The histological observation showed that MVD was significantly highe

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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