[Effects of adipose stem cell-derived exosomes on rat tendon healing and its impact on the periphery neuropeptides expression].

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective: To investigate the effect of injectable adipose tissue stem cell-derived exosome-encapsulated hydrogel on the tendon healing in rats and to evaluate the temporal and spatial expressions of periphery neuropeptides at healing site. Methods: To generate the injectable exosome-encapsulated hydrogel, the methacryloylchloride solution (GelMA) and the photoinitiator were mixed first, then the exosome solution was added and oscillated together. Followed with exposing in the ultraviolet light in a wave length 405 nm for 30 seconds to form an injectable hydrogel. The Sprague Dawley (SD) rats were performed with full thickness transection and surgical repair administration of Achilles tendon to establish the animal model, including 4 groups: intact control (C group, selected the contralateral side of tendon transection with surgical repair group), tendon transection with surgical repair group (S group), tendon transection with surgical repair and hydrogel implantation group (H group) and tendon transection with surgical repair and exosome-encapsulated hydrogel group (E group). The samples were harvested on Day 7, 14 and 28 after the operation, respectively. Histopathological examination was performed with hematoxylin-eosin staining (HE staining) and immunohistochemistry staining of tenogenesis marker (Tenomodulin, TNMD), periphery neuropeptide markers (growth associated protein

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

Browse all related research

Filter the research library by this study's title keywords, author, or publication year.