Effects of Local and Systematic Administration of Adipose Tissue-Derived Stem Cells to Intestinal Anastomosis in Intestinal Ishemic Rerfusion Injury: Ani̇mal Experi̇ment Model.

Several human studies show positive signals, while research methods and sample sizes continue to develop.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Intestinal ischemia-reperfusion (IR) injury compromises anastomotic healing and may weaken anastomotic integrity. Adipose tissue-derived stem cells (AD-SCs) have been shown to enhance tissue regeneration through angiogenic and anti-ischemic effects. This study aimed to evaluate the impact of local and systemic AD-SC administration on anastomotic healing and strength following intestinal IR injury using histopathological parameters and bursting pressure measurements. Thirty male Wistar Albino rats were randomly assigned to three groups (n = 10 each): control, local AD-SC, and systemic AD-SC. IR injury was induced by clamping the superior mesenteric artery, followed by intestinal resection and anastomosis. Local or systemic AD-SCs (1 × 10 6 cells in 1.5 mL phosphate-buffered saline) were administered according to group allocation. On postoperative day 7, serum hydroxyproline levels, bursting pressures, and semiquantitative histological scores (ischemic necrosis, vascular proliferation, re-epithelialization, and collagen density) were assessed. Ordinal histological outcomes were analyzed using Kruskal-Wallis tests with Dunn's post hoc comparisons. Six animals died before the end point, leaving 24 rats for analysis. Bursting pressures and serum hydroxyproline levels did not differ significantly among groups. However, systemic AD-SC administration significantly improved all histolog

Evidence level

Several human studies show positive signals, while research methods and sample sizes continue to develop.

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