Level D· Preclinical EvidenceLaboratory Study

Efficacy of the combination of umbilical fat and venous blood on limb salvage.

Hafiz E.

Laboratory Study with a reported sample of 20 on Ankle Injury, Peripheral Artery Disease, published in Front Bioeng Biotechnol (2026) — summary generated from the PubMed abstract.

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Study type
Laboratory Study
Journal
Front Bioeng Biotechnol (2026)
Country
Switzerland
Reported sample size
20
PMID
41822465
DOI
10.3389/fbioe.2026.1775975

Abstract (original English)

To evaluate the efficacy of combined umbilical adipose tissue and venous blood-derived stromal vascular fraction (SVF) on limb perfusion, pain and functional recovery in patients with chronic limb-threatening ischemia (CLTI). A total of 20 patients aged between 36 and 82 years (mean age: 60.3 ± 11.2 years) were included in the study. Ankle-Brachial Index (ABI), pain scores and pain-free walking distance were evaluated before treatment and at follow-up at 3, 6, 9 and 12 months. At 12 months, ABI increased significantly, Visual Analog Scale (VAS) decreased, while pain-free walking distance improved at 9 and 12 months compared to baseline. Left ventricular ejection fraction (LVEF) showed a non-significant rise (54.6%-55.2%, p = 0.072), while renal function improved modestly (creatinine 1.12 → 1.09 mg/dL, eGFR 78 → 80.5 mL/min/1.73 m 2 , p = 0.034). Angiography revealed enhanced vessel visualization and collateral formation. Combined Autologous conditioned plasma (ACP) and adipose-derived SVF injections effectively improved limb perfusion, pain, lesion severity and functional outcomes in CLTI patients, suggesting a promising therapeutic approach for peripheral arterial disease (PAD).

What this study does not prove

  • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

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