Efficacy of mesenchymal stem cells cultured low oxygen tension ameliorates apoptotic inhibitors, viability, and differentiation of ovarian tissue: A study in a rat model with ovarian failure

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background In cases of ovarian failure due to malnutrition, stem cell therapy is ineffective due to the lack of viability and differentiation of transplanted cells, resulting in low adaptation and survival rates. This study aimed to investigate whether stem cells cultured under low oxygen (O 2 ) tension improve the adaptability and viability of cells in rats with ovarian failure. Methods After four days of culturing mesenchymal stem cells (MSCs) in 21% oxygen (normoxia) as the T2 group and 1% oxygen (low O 2 or hypoxia) as the T1 group, 200 million bone marrow-derived MSCs per rat were transplanted into female rats with ovarian failure (15 rats per treatment group). A total of 15 fertile and 15 infertile rats were categorized as the C+ and C- groups, respectively. Results The slight increase gradually in cells expressing HSP70, VEGF-1, and GDF-9 was reported in C+, T2, T1, and C- groups, respectively. Caspase-3 expression was reported to decrease gradually in C+, T2, T1, and C- groups. Meanwhile, Graafian follicle count was reported to increase gradually in C+, T2, T1, and C- groups, respectively. Conclusions Based on those parameters, MSCs cultured under low O 2 tension were more effective than those cultured under normoxic conditions as a treatment for female rats with ovarian failure.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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