Efficient Isolation of Adipose-derived Stem Cells and Adipocytes from Porcine Adipose Tissue.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Adipose-derived stem cells (ADSCs) have emerged as ideal seed cells in regenerative medicine due to their abundant sources, minimally invasive harvesting, multi-lineage differentiation potential, and immunomodulatory properties. Their applications span tissue repair, disease modelling, and cell therapy; however, efficient isolation of high-viability ADSCs remains critical for advancing research and clinical translation. Conventional isolation methods, such as enzymatic digestion combined with mechanical dissociation via pipetting, are limited by lengthy processing times (1-3 h), poor cell viability (often <70%), and substantial batch-to-batch variability, compromising downstream experiments. Here, we present the SoniConvert system (a mechanical wave-based cell separation system), which combines mechanical wave and enzymatic digestion to address these challenges. The system integrates a microprocessor-based control unit that regulates mechanical wave-mediated dissociation via digital feedback, together with a tissue-specific loosening reagent optimized for adipose tissue (5-15 min incubation). This approach enables rapid conversion of tissue to a single-cell suspension, with mechanical dissociation completed in 3-9 s. The system offers three advantages: (1) ultra-fast processing, with the core isolation process-from enzymatic digestion to initial cell fraction separation-complet

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

Browse all related research

Filter the research library by this study's title keywords, author, or publication year.