Engineering bone from fat: a review of the in vivo mechanisms of adipose derived stem cell-mediated bone regeneration.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Adipose-derived stromal/stem cells (ASCs) have considerable potential to promote bone regeneration due to their ease of isolation, abundance, and osteogenic capacity. However, despite two decades of research, studies of the precise mechanisms underlying their in vivo osteo-regenerative capacity remain contradictory. Specifically, there are multiple reports that suggest either a direct mechanism (i.e. ASCs directly differentiate into osteoblasts and lay done new bone matrix) or an indirect mechanism (i.e. ASCs stimulate endogenous cells via their secretory profiles) of bone regeneration. To address this discrepancy we critically reviewed studies utilizing ASCs for in vivo bone regeneration and employed methods to track cell fate. We evaluated the rigor of individual studies by examining the specific defect and animal models employed, cell sorting or pretreatment methods, and quantitative assessments of cell dosing and cell survival/distribution data to determine the strength of their claims. There is robust evidence to support both a direct differentiation of ASCs and indirect signaling based on secreted factors: osteogenic/angiogenic, immunomodulatory, or extracellular matrix factors, suggesting that a combination of factors underlie the pro-regenerative capacity of ASCs. However, there remains significant knowledge gaps regarding the precise efficiency of ASC engraftment follo

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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