Establishing protocols for the efficient expansion of canine and feline adipose tissue-derived mesenchymal stromal cells following cell isolation.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

BACKGROUND: Novel treatments using autologous or allogeneic mesenchymal stromal cells (MSC) are gaining traction in both human and veterinary medicine. MSC secrete multiple factors with immunomodulatory properties, making them promising for treating immune-mediated and inflammatory conditions. However, their application is challenged by donor variability, inconsistent tissue sources and lack of standardized manufacturing protocols, especially in veterinary medicine approaches exploiting the use of canine and feline MSC. Adipose tissue (AT), in particular, is a readily accessible MSC source for both species. This study applied a previously established enzymatic isolation method and focused on developing an efficient ex vivo expansion protocol for canine and feline adipose tissue-derived MSC, emphasizing the selection of a cost-effective culture medium that ensures high viability and proliferation. Subcutaneous AT was collected from 5 female dogs and 5 female cats during ovariohysterectomy. Samples were processed enzymatically, and isolated cells were cultured in basal medium supplemented with different concentrations of fetal bovine serum (FBS) (either standard FBS or FBS pre-screened for MSC-culture, hereon FBS-MSC). MSC identity was confirmed by flow cytometry (CD90+, CD44+, MHC-II−) and multilineage differentiation assays. RESULTS: Upon isolation, cells showed typical MSC mor

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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