Evaluating the Homing Potential of Adipose-Derived Stromal Cells Following Dorsal Subcutaneous Injection in a Rat Fat Graft Model.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Cell-assisted lipotransfer is a fat-grafting technique that uses adipose tissue from lipoaspirates mixed with adipose-derived stromal cells (ASCs) to enhance fat graft survival. Intravenously injected ASCs migrate to the fat graft site via circulatory system; however, a large portion of circulatory ASCs can get trapped in the lungs, reducing the amount of available ASCs for fat graft. The molecular and cellular mechanisms modulating ASC homing remain unclear. We aimed to investigate the migration and infiltration of ASCs to adjacent fat graft when injected subcutaneously. Methods A fat graft was implanted on the dorsal side of Sprague-Dawley rats without genetic modification. Green fluorescent protein (GFP)-ASCs were harvested from genetically engineered Sprague-Dawley rats and injected subcutaneously cephalically away from the fat graft at 0, 1, and 2 cm. The migration and fate of the injected GFP-ASCs in relation to fat grafts were monitored using an in vivo imaging system for 4 weeks, and GFP fluorescence in fat graft was examined under a microscope. Results Cell migration was observed at both 1 and 2 cm from the fat graft. However, ASC infiltration to the fat graft was greater at a distance of 1 cm away from the graft than at 0 and 2 cm. Conclusion ASC migration into fat grafts was observed after subcutaneous injection. ASCs injected at a distance of 1 cm showed

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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