Exosomes from adipose-derived stem cells preconditioned with 650- or 810-nm photobiomodulation enhance cutaneous wound healing in rats.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

This study investigated whether photobiomodulation (PBM) preconditioning at 650 nm or 810 nm modifies the regenerative potential of exosomes secreted by human adipose-derived stem cells (HADSCs) during full-thickness acute skin wound healing in rats. Although both wavelengths have been shown to enhance ADSC viability, proliferation, and exosome release in vitro, their in vivo reparative efficacy remains incompletely defined. We had 4 groups: untreated controls, animals treated with exosomes from non-preconditioned HADSCs, and two groups receiving exosomes derived from HADSCs exposed to either 650-nm or 810-nm PBM. Exosomes were administered at a concentration of 150 μg/mL and a total dose of 450 μg per animal. Wound tissues were harvested on day 8 for biomechanical testing, stereological evaluation of tissue components, and analysis of miR-21, VEGF-A, and HIF-1α expression. The EXO 650, and EXO 810 groups demonstrated 119%, and 105% increases in Stress High Load capacity compared to control group (both p < 0.001). The EXO 650 and EXO 810 wavelengths resulted in 58% and 67% increase in fibroblast population (p < 0.01, p < 0.001). EXO 650 and EXO 810 wavelengths showed 43%, and 64% increases in the number of new blood vessels (p < 0.01, p < 0.001) over control values. Molecular signaling analysis via fold-change quantification indicates that Exo 650 and Exo 810 wavelengths achiev

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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