[An experimental study on repairing full-thickness skin wound by human acellular amniotic membrane loaded with adipose-derived stem cells in rats].

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Human acellular amniotic membrane (HAAM) contains collagens, glycoproteins, protein-polysaccharide, integrin, and lamellar, which can supply rich nutrition to cell proliferation and differentiation. To explore the possibility of HAAM with adipose-derived stem cells (ADSCs) as a good engineered skin substitute for repairing skin defect. Primary ADSCs were obtained from inguinal fat of 30 healthy 4-month-old SD rats, male or female, weighing 250-300 g, and cultured in vitro and purified. The 3rd passage ADSCs were used to detect CD44, CD49d and CD34 by immunocytochemistry staining. After physical and trypsin preparation, the HAAM was observed by HE staining and scanning electron microscope(SEM) respectively. ADSCs were seeded on epithelial side of HAAM at the density of 2 x 10(5)/cm2, cocultured, and observed by SEM at different time. MTT test was used to detect viability of cells that seeded on HAAM, the group without HAAM was used as control. Thirty SD rats were made models of full-thickness skin wound and randomly divided into three groups (A, B, and C). Wound was repaired with HAAM/ADSCs composites in group A, with HAAM in group B, and with gauze as control in group C. The rats underwent postoperative assessment of wound healing rate and histological observation at the 1st, 2nd, and 4th weeks. HE staining showed that the 3rd passage ADSCs was spindle-shaped with an ovoid nucl

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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