SVF
Level D· Preclinical EvidenceAnimal Study

Experimental study on the in vitro osteogenic and chondrogenic ability of fat stem cells combined with 3D-printed porous scaffolds.

He Y., Li X., Tu Z., Chen H., Zeng H., Peng Q.

Animal Study on Osteoarthritis, Cartilage Damage, Hip Osteoarthritis, published in BMC Musculoskelet Disord (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
BMC Musculoskelet Disord (2026)
Country
England
Reported sample size
—
PMID
41547791
DOI
10.1186/s12891-025-09476-0

Abstract (original English)

Developmental Dysplasia of the Hip (DDH) is an acetabular deformity caused by stress concentration or abnormal stress during weight-bearing, and it constitutes a significant etiological factor for secondary hip osteoarthritis. Currently, there remains controversy surrounding the selection of treatment protocols for DDH. This study aims to systematically evaluate the osteogenic and chondrogenic differentiation capacities of adipose-derived stem cells (ADSCs) seeded on 3D-printed porous polycaprolactone (PCL) scaffolds in vitro, thereby opening up a new avenue for addressing osteochondral tissue defects and providing a more effective and safe therapeutic approach for patients with developmental dysplasia of the hip. Rat ADSCs were seeded onto fabricated 3DPPCL scaffolds. The scaffolds' biocompatibility and support for cell adhesion were confirmed. ADSCs were then cultured under osteogenic or chondrogenic conditions. Differentiation was assessed using Alizarin Red and Alcian Blue staining for mineralization and glycosaminoglycan (GAG) deposition, respectively, RT-qPCR for key gene markers (e.g., RUNX-2, COL-II), and immunofluorescence for protein expression. Experimental groups included scaffolds modified with different concentrations of dopamine or chondroitin sulfate. The 3DPPCL scaffolds supported robust ADSC adhesion and proliferation. Osteogenic induction significantly enhanc

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
AnimalsTissue ScaffoldsOsteogenesisChondrogenesisPrinting, Three-DimensionalRatsPorosityCells, CulturedCell DifferentiationTissue Engineering
HipOsteoarthritisCartilage DamageHip Osteoarthritis
View on PubMedDOI

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