Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

To investigate the hormonal regulation of P450 aromatase activity (responsible for the conversion of C19 androgens to C18 oestrogens) in human adipose tissue from men and pre- and post-menopausal women. Subcutaneous abdominal adipose tissue was obtained from 19 subjects: six pre-menopausal females (mean age 41.8+/-(s.e.m.) 2.5; mean weight 76.01+/-5.6 kg), eight post-menopausal females (mean age 59.9+/-2.0; mean weight 63.5+/-2.6 kg), and five males (mean age 35.8+/-8.8; mean weight 78.5+/-7.8 kg) undergoing elective or cosmetic surgery. Cell viability and cell size were determined using staining techniques. RT-PCR was used to confirm the presence of aromatase. The regulation of aromatase activity was characterized using androstenedione as a substrate in a tritiated water release assay. Aromatase activity was analysed in abdominal subcutaneous stromal cells (ASC) and mature adipocytes (AD) cultured in serum-free medium with cortisol (10-6-10-7 M), insulin (500 nM) or a combination of both. In ASC aromatase activity increased in females from 14.5+/-1.7 to 29. 3+/-2.6 pmol/mg/h (n=14, P<0.05) and to 25.2+/-2.1 pmol/mg/h with cortisol (10-7 M) and insulin, respectively (P<0.05). In males ASC basal aromatase activity (20.5+/-4.2 pmol/mg/h; n=5) was inhibited by cortisol (10-7 M) alone (12.3+/-1.8 pmol/mg/h) and in combination with insulin (6.6+/-1.2 pmol/mg/h; men vs women, P<0.005

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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