[High-dose neuropeptide Y inhibits proliferation and promotes differentiation of human adipose-derived stem cells].

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective To investigate the impact of neuropeptide Y (NPY) at different concentrations on the proliferation and differentiation of human adipose-derived stem cells (hADSCs). Methods The hADSCs were separated and incubated with NPY at a range of concentrations 10(-6)-10(-15) mol/L. MTT assay was used to detect cell proliferation. Following the treatment of complete medium, NPY and insulin separately, cell differentiation was observed by Oil red O staining. In addition, Western blotting was performed to exam the levels of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), cell death-inducing dFF45-like effector c (Cidec) and receptor interacting protein 140 (RIP140). Results MTT assay revealed that 10(-11)-10(-15) mol/L NPY stimulated the proliferation of hADSCs, while high-dose NPY (10(-6)-10(-10) mol/L) inhibited the cell proliferation. NPY (10(-7), 10(-9), 10(-11) mol/L) induced the differentiation of hADSCs. The expression levels of related adipocyte markers (PPARγ, C/EBPα), mature white adipose tissue specific markers (Cidec, RIP140) increased at the presence of NPY (10(-7), 10(-9), 10(-11) mol/L). Conclusion Low-dose NPY could stimulate the proliferation of hADSCs, and high-dose NPY could inhibit the proliferation and promote the differentiation of hADSCs.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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