A high throughput microfluidic system with large ranges of applied pressures for measuring the mechanical properties of single fixed cells and differentiated cells

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

The mechanical properties of cells are of great significance to their normal physiological activities. The current methods used for the measurement of a cell's mechanical properties have the problems of complicated operation, low throughput, and limited measuring range. Based on micropipette technology, we designed a double-layer micro-valve-controlled microfluidic chip with a series of micropipette arrays. The chip has adjustment pressure ranges of 0.03-1 and 0.3-10 kPa and has a pressure stabilization design, which can achieve a robust measurement of a single cell's mechanical properties under a wide pressure range and is simple to operate. Using this chip, we measured the mechanical properties of the cells treated with different concentrations of paraformaldehyde (PFA) and observed that the viscoelasticity of the cells gradually increased as the PFA concentration increased. Then, this method was also used to characterize the changes in the mechanical properties of the differentiation pathways of stem cells from the apical papilla to osteogenesis.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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