Human umbilical cord mesenchymal stem cell delivery of mitochondria to melanocytes enhances skin repigmentation efficacy in autologous epidermal cell suspension transplantation through the TNFAIP2-TNT system

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Autologous epidermal cell suspension (AECS) transplantation is essential for treating large-area depigmenting diseases and some chronic wounds that are refractory to conservative therapy. However, this technique encounters challenges, including limited repigmentation efficacy and high costs, which have hindered its clinical adoption. To enhance the repigmentation efficacy of AECS, our research explored the optimal conditions for preparing suspensions of epidermal cells (ECs). Next, we investigated the functions of melanocytes (MCs) and repigmentation efficacy through co-culturing and co-transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) with AECS. The addition of hUCMSCs to this system enhanced the proliferation, migration, and melanin synthesis capabilities of the ECs. These findings were validated in vivo , with the hUCMSCs co-transplantation group demonstrating superior skin repigmentation efficacy in mice. Then gene interference, overexpression, and transcriptome analyses were conducted to elucidate the mechanisms underlying the Tumor Necrosis Factor Alpha-Induced Protein 2 (TNFAIP2)-Tunneling Nanotubes (TNTs) system in mediating melanin synthesis. Our findings outline a novel pathway through which hUCMSCs, via the TNFAIP2-TNT system, mediate the delivery of mitochondria to melanocytes, enhancing the function of MCs. This presents new avenues for impro

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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